Year: 2021 | Month: December | Volume 11 | Issue 6
Genetic Diversity Studies using Microsatellite Markers to Analyse Genetic Variation among the Buffalo Breed Populations in Jammu Region
S Gurdeep Singh
Amitoz Kour
R.K. Taggar
D. Kumar
D. Chakarborty
DOI:10.30954/2277-940X.06.2021.8
Abstract:
The microsatellites are the markers of choice for similar genetic exploration in different buffalo breeds both in India and abroad. In the present study, the molecular genetic characterization of local germplasm within and between existing buffalo population carried out using microsatellite markers with the objectives of studying the molecular characteristics of these buffaloes and to study available genetic diversity in the existing breed population. Fifty (50) and Twenty five (25) venous blood samples were collected at random from genetically unrelated animals of different sex and age groups of local buffalo gemplasm from its natural breeding tract in J&K region. The PCR products for different microsatellite loci were resolved on 6 percent denaturing (urea) polyacrylamide gels along with 50 and 100 bp DNA ladders at 40-45W. Microsatellite alleles were visualized by silver staining. The microsatellite genotype data were analyzed using POPGENE version. The genetic distance (Ds) between two breeds as calculated according to Nei’s standard genetic distance revealed Nei’s genetic identity and genetic distance to be 0.8038 and 0.2184 respectively between local buffalo population and Murrah breed which shows suggests a close relationship between Murrah and local buffaloes as expected from their geographical contiguity although they are phenotypically distinct. Present findings may be useful in characterization of genetic diversity and to develop strategies for conservation and utilization of local germplasm of J&K region, within and between existing buffalo population. The two studied buffalo population in present study has shown genetically distinctness from each other.
Highlights
- Closeness of Local buffalo population (Gujjari buffalo) and Murrah breed on the basis of Nei’s measures as expected from same geographical contiguity.
- All the 15 microsatellite loci, amplified successfully were found to be polymorphic in local Buffaloes.
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